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solid-phase peptide synthesis vessel  (Chemglass)

 
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    Structured Review

    Chemglass solid-phase peptide synthesis vessel
    Solid Phase Peptide Synthesis Vessel, supplied by Chemglass, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/solid+phase+peptide+synthesis+vessel/peptide+synthesis+vessel/pm37078604-217-20-29
    Average 90 stars, based on 1 article reviews
    solid-phase peptide synthesis vessel - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Chromatography:

    Article Title: Specificity Profiling of Protein-Binding Domains Using One-Bead-One-Compound Peptide Libraries
    Article Snippet: .. Materials Chemglass* Solid Phase Peptide Synthesis Vessel with Inner Luer Joint > 25 mL; Disc O.D: 20 mm; GL size: 25 ( {"type":"entrez-nucleotide","attrs":{"text":"CG186202","term_id":"34077263","term_text":"CG186202"}} CG186202 , Chemglass No.:CG-1862-02) list-behavior=simpleprefix-word=mark-type=nonemax-label-size=0 Part A Materials list-behavior=simpleprefix-word=mark-type=nonemax-label-size=0 N’N’-DimethylFormamide (Sigma Aldrich) Dichloromethane (Sigma Aldrich) Bio-Spin Dispoable Chromatography Columns (BioRad cat # 732-6008) TentaGel S NH 2 resin (90 μm, 0.3 mmol/g) O-Benzotriazole-N,N,N’,N’-tetramethyluronium hexafluorophosphate (HBTU) (Aapptech) N-Hydroxybenzotriazole (HOBt) (Aapptech) Diisopropylethylamine (DIPEA) DCM/Ethyl Ether (55:45 v/v) Fmoc-Met-OSu (Aapptech) Fmoc-Met-OH (Aapptech) Boc-Phe-OSu (Aapptech) 50% (v/v) trifluoroacetic acid (TFA)/DCM 2% acetaldehyde in DMF and 50 μL of 2% chloranil in DMF Part B Materials: list-behavior=simpleprefix-word=mark-type=nonemax-label-size=0 All Fmoc-Amino Acids desired with acid-liable sidechain protecting groups (tBu, Boc etc) (Aapptech) HBTU. .. HOBT, DIPEA (see above) 20% Piperidine in DMF 5 g ninhydrin in 100 mL of ethanol, 80 g phenol in 20 mL of ethanol, 1 mM KCN in 98% pyridine Part C Materials: list-behavior=simpleprefix-word=mark-type=nonemax-label-size=0 All materials from previous section with the addition of CD 3 CO 2 D (Sigma-Aldrich) CH 3 CD 2 CO 2 D (Sigma-Aldrich) Part D Materials: list-behavior=simpleprefix-word=mark-type=nonemax-label-size=0 Fmoc-pY-OH (Aapptech) HATU, DIPEA (Aapptech) Acetic anhydride (Sigma Aldrich) Part E Materials: list-behavior=simpleprefix-word=mark-type=nonemax-label-size=0 Modified reagent K: (6.5% phenol, 5% water, 5% thioanisole, 2.5% ethanedithiol, 1% anisole, and 1% Triisopropylsilane in TFA) 20% piperidine in DMF list-behavior=enumeratedprefix-word=mark-type=upper-alphamax-label-size=0 Bead Segregation and Surface Density Reduction This protocol decreases the peptide loading in the surface layer of each bead and greatly reduces the number of false positive beads due to nonspecific binding during library screening. list-behavior=simpleprefix-word=mark-type=nonemax-label-size=1 1 Soak the resin (5 g) in water overnight.

    Incubation:

    Article Title: Solid-phase synthesis of protein-polymers on reversible immobilization supports
    Article Snippet: .. A volume of 4.5 mL of protein solution (2 mg mL −1 , 100 mM sodium phosphate (pH 6 or 8) containing 0.05 v/v% Tween 20 was combined with 1.5 mL of DMA beads in the solid phase peptide synthesis vessel (10 mL capacity, Chemglass) and shaken at room temperature or in refrigerator for 30 min. After removing the supernatant, the beads were washed with incubation and washing buffers. .. The amount of immobilized protein on the DMA beads was determined using a Micro BCA Protein Assay Kit (ThermoFisher Scientific).



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